Agilent liquid chromatography column AdvanceBio Desalting RP Introduction:

Used for online removal of salt ions before mass spectrometry detectionDesalination reverse column
Affinity, ion exchange, and volume exclusion chromatography are commonly used techniques for analyzing proteins such as monoclonal antibodies. However, these technologies require an aqueous mobile phase containing non-volatile salts. When using mass spectrometry for detection, these non-volatile salts can cause signal suppression and salt deposition can contaminate the mass spectrometer detector, resulting in increased maintenance requirements and prolonged instrument downtime. AdvanceBio desalination reverse phase column solves this problem and can quickly and efficiently remove salt ions online before mass spectrometry detection. This type of small column chromatography column can be used alone in any liquid chromatography system to remove salts from collected fractions, or it can also be used as the second dimension of the Agilent 1290 Infinity II two-dimensional liquid chromatography system for desalination after separation in the first dimension.

Agilent liquid chromatography column AdvanceBio Desalting RP characteristic

• Enable traditional non mass spectrometry friendly technologies such as IEX, SEC, and affinity chromatography to be detected using mass spectrometry
• Online desalination of samples effectively saves time
• Enrich the sample before elution
• Keeping the mass spectrometry cleaner reduces downtime and minimizes maintenance
• Improve data quality to give you full confidence in overall data
• Featuring a large particle size of 1000 Å, suitable for large biomolecules
• Stable within the pH range of 2-13
• Compatible with all commonly used reverse flow phases

Agilent liquid chromatography column AdvanceBio Desalting RP